DynamiX
MICROPHYSIOLOGICAL FLUX ANALYZER
BENEFITS
Real-Time Data Tracking
OPERATION
How DynamiX Works
1
Sensor plate loaded
with organoids
2
Automated organoid
inclusion/exclusion
3
Dynamic screen of
metabolic function
4
Cloud-based
data analysis
APPLICATIONS
Unravel Mechanisms of Action
Cancer Biology
Metabolic Disease
Viral Infection
Drug Safety Analysis
DynamiX permits tracking the dynamics of central carbon metabolism in real time. Rapid non-destructive measurements were used to identify an idiopathic CYP450-independent mechanism of acetaminophen¹. Explain the post-approval market withdrawal of troglitazone². Show a novel mechanism of drug-induced liver toxicity of valproate³. Expand the safety margin of cisplatin with combination therapy, followed by clinical validation⁴. Expand the safety margin of gentamycin⁵. Identify novel pathways in COVID-19 infection⁶ and novel mechanisms of human arrhythmia⁷.
1. Prill, et al. Arch Toxicol. 90(5):1181-91. (2016) | 2. Bavli, et al. PNAS 113(16):E2231-40. (2016) | 3. Ehrlich, et al. Lab Chip. 21;18(17):2510-2522 (2018). | 4. Cohen, et al. Science Transl Med. 13(582). (2021) | 5. Ioannidis, et al. Lab Chip. 22(23):4469-4480 (2022). | 6. Ehrlich, et al. eLife 12 (2023). | 7. Ghosheh, et al. Nature Biomedical Engineering (7): 1493-1513 (2023)
Integrates automation-friendly technologies
Avoids manual and error‑prone processes
Tracks key metabolic aspects for weeks in real time without human intervention
Provides thousands of human‑relevant datapoints from each experiment
Suits both independent machine learning and integrated in silico-in vitro approaches.
Extends safety and efficacy evaluations far beyond current methodologies
AUTOMATED DEPOSITION
Our technology relies on Robot Directed Organoid Deposition (RODEO). A 1.1µL microdroplet containing a mixture of cells in a basement membrane matrix is deposited in a well. Each microdroplet supports the growth of a single organoid, with embedded oxygen microsensors that don't affect their viability. This method yields a high formation rate (>94%), consistent organoid size and low variability. Current organoid methodologies consist on microsection or stem cell expansion in a three-dimensional matrix. They result in higher variability in organoid quantity, size and composition, as well as inter-assay and intra-assay variance.
SOFTWARE
MechaniX Capabilities
EXCLUSION CRITERIA
MechaniX software suite was built for high throughput screening (HTS) applications. The software tracks organoid size and location in real-time. The MechaniX quality control tool allows users to exclude malformed organoids of specific sizes or shapes. The software also allows users to select metabolic active organoids (e.g., terminally differentiated) based on their metabolic activity, limiting assay variability.
ENHANCED EXPERIMENT DESIGN
MechaniX software suite supports complex experimental design and cloud integration. The dynamic data is uniquely suited for machine learning applications and integrated in silico-in vitro approaches. End-point analysis tools allow users to replicate traditional screening analyses like TC₅₀ /EC₅₀ values. Dose over time kinetic (DoTK) analysis opens dynamic features such as time to onset (TTO) and toxicity thresholds as a function of treatment duration (Tₜₕ) which are equivalent to NOAEL.
