Unique Cell Library
Tissue Dynamics utilizes an ethnically diverse (European, Hispanic, Asian) library of proliferating human hepatocyte lines that show minimal experimental variability. Metabolic activity and toxicological profile were extensively validated.
3D Physiological Organs
Proprietary Organ on Chip design allows us to form and maintain 3D liver tissue for over 28 days. Micro tissues acquire physiological structure and show complex metabolic zonation.
Embedded Sensors Feel Cell Stress
Tissue Dynamics patent-protected technology permits the real time assessment of metabolic function. Change in metabolic function is the first indication of cellular stress, preceding manifestation of any detectable damage.
Lack of metabolic stress is an excellent indicator of clinical safety
Rate and direction of metabolic changes provide mechanistic insight to toxicity
Y. Avior, G. Levy, M. Zimerman D. Kitsberg, R. Schwartz, R. Sadeh, A. Moussaieff, M. Cohen, J. Itskovitz-Eldor, Y. Nahmias. Microbial-Derived Lithocholic Acid and Vitamin K2 Drive the Metabolic Maturation of Pluripotent Stem Cells Derived Hepatocytes. Hepatology 2(1): 265-78. (2015)
G. Levy, D. Bomze, S. Heinz, S.D. Ramachandran, A. Noerenberg, M. Cohen, O. Shibolet, E. Sklan, J. Braspenning, Y. Nahmias. Long-term culture and expansion of primary human hepatocytes. Nature Biotechnology 33(12): 1264-1271. (2015)
S. Prill, D. Bavli, G. Levy, E. Ezra, E. Schmälzlin, M.S. Jaeger, M. Schwarz, C. Duschl, M. Cohen, Y. Nahmias. Real-time monitoring of oxygen uptake in hepatic bioreactor shows CYP450-independent mitochondrial toxicity of acetaminophen and amiodarone. Arch Toxicol. 90(5):1181-91. (2016)
D. Bavli, S. Prill, E. Ezra, G. Levy, M. Cohen, M. Vinken, J. Vanfleteren, M. Jaeger, Y. Nahmias, Real-time monitoring of metabolic function in liver-on-chip microdevices tracks the dynamics of mitochondrial dysfunction. PNAS 113(16):E2231-40. (2016)
A. Ehrlich, S. Tsytkin-Kirschenzweig, K. Ioannidis, M. Ayyash, A. Riu , et al. Microphysiological flux balance platform unravels the dynamics of drug induced steatosis. Lab on a Chip 18:2510-22 (2018)